![]() ![]() Here we summarise the EV preparation protocols for AFM and EM bringing out the main challenges in the imaging of EVs, both in their natural environment as biofluid constituents and in a saline solution after EV isolation. However, a prerequisite for such usage of EVs is their detailed characterisation, including visualisation which is mainly achieved by atomic force microscopy (AFM) and electron microscopy (EM). Because EV composition reflects the state of its parental tissue, EVs possess an enormous diagnostic/prognostic potential to reveal pathophysiological conditions. This event was developed under the patronage of the International Federation for the Promotion of Mechanism and Machine Science (IFToMM), through the Technical Committee Sustainable Energy Systems.Extracellular vesicles (EVs) are nanometric membranous structures secreted from almost every cell and present in biofluids. The sub-chapters of the book were openly presented during the 4 th Edition of the Conference for Sustainable Energy, held 6-8 November, 2014 and organized by the R&D Centre Renewable Energy Systems and Recycling at the Transilvania University of Brasov, Romania. The need for new strategies and implementation tools, for education and training is addressed in the final chapter as part of the nZEB concept, towards sustainable communities. The concept is discussed further in a chapter on advanced solutions for water re-use and recycling wastes as second raw materials. These two chapters discuss the problem of conversion efficiency at the level of components and systems, aiming at giving value to the variable renewable energy sources, in producing thermal and electric energy. ![]() Advanced solutions to cover the energy needs by using various renewable-based energy mixes are presented in two chapters. ![]() The book starts with a chapter that addresses problems related to the energy demand and renewable energy sources available in the built environment, along with the restrictions and opportunities in developing sustainable, efficient and affordable solutions, also gaining aesthetic and architectural acceptance. This book addresses the main challenges faced today in implementing the Nearly Zero Energy Buildings (nZEB) concept. ![]()
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